ANA by IFA, Reflex to 9-biomarker profile, dsDNA, RNP, Sm, SS-A, SS-B, Scl-70, Chromatin, Jo-1, Centromere B by Multiplex Immunoassay
Also known as: ANA IFA Reflex
Use
Assay for screening for ANAs in patients suspected of having connective tissue disease.
Special Instructions
If reflex testing is performed, additional charges/CPT code(s) may apply according to specific biomarker profiles.
Limitations
Males and females older than 80 years of age have a 50% incidence of low titer ANA. The test may report positive due to certain medications that induce a 'lupoid' condition; however, the titer usually decreases following the withdrawal of the drug. Additionally, low antibody levels may also be present in conditions other than connective tissue diseases. A negative test is strong evidence against SLE, but it is not conclusive.
Methodology
Immunoassay (Multiplex Protein Panel)
Biomarkers
LOINC Codes
- 42254-3
- 77202-0
- 20398-4
- 20399-2
- 20401-6
- 5077-3
- 53003-0
- 54006-2
- 54155-7
- 53998-1
- 53006-3
- 53993-2
- 8251-1
- 5130-0
- 29374-6
- 11090-8
- 27416-7
- 17792-3
- 17791-5
- 51775-5
- 11565-9
- 53982-5
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.5 mL
Container
Red-top tube or gel-barrier tube
Storage Instructions
Room temperature
Causes for Rejection
Hemolysis; lipemia; gross bacterial contamination
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 7 days |
| Frozen | 7 days |