ANA 12 Plus Profile (RDL)
Also known as: ANA 12 Plus, ANA 12 Plus Panel
Use
The ANA 12 Plus Profile is a reflexive assay. If the ANA titer is negative, no further tests are performed except for Anti-Ro Ab. Anti-Ro is performed to find the very small percentage of false negative ANAs. If the ANA titer is positive, the complete profile is performed. The ANA test detects autoantibodies that bind to the nuclear components of HEp-2 cells. A positive ANA titer is not specific, but indicates the presence of one or more autoantibodies. Profiles of specific autoantibodies have diagnostic and potential prognostic utility.
Special Instructions
If the antinuclear antibodies titer is positive, all tests listed are performed. If the antinuclear antibodies titer is negative, an Anti-Ro (SS-A) Ab test is performed to catch the small percentage of false negative antinuclear antibodies results. The ANA includes titer and pattern specifications.
Limitations
A positive antinuclear antibodies titer is not specific and indicates the presence of one or more autoantibodies, which may not be indicative of any specific disease. Additional confirmatory or reflex testing may be required in some cases, potentially extending the turnaround time.
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 42254-3
- 42254-3
- 33569-5
- 5077-3
- 47299-3
- 11090-8
- 57662-9
- 45142-7
- 26975-3
- 5348-8
- 3181-5
- 5076-5
- 3182-3
- 4485-9
- 4498-2
- 8099-4
- 32580-3
- 57093-7
- 11572-5
Result Turnaround Time
7-10 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
5 mL
Minimum Volume
4 mL
Container
Red-top tube or gel-barrier tube
Collection Instructions
Separate serum from cells within one hour of collection. Transfer to a plastic transport tube before shipping.
Storage Instructions
Refrigerate.
Causes for Rejection
Grossly hemolyzed, bacterial contamination, lipemic, icteric, non-serum specimen types
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 1 day |
| Refrigerated | 8 days |
| Frozen | 8 days |