Androstenedione, LC/MS
Also known as: 4-Androstenedione, δ4-Androstene,3,17-dione
Use
Androstenedione is a 19-carbon steroid hormone produced in the adrenal glands and gonads. It serves as a precursor in the biochemical pathway producing the androgen testosterone and estrogens estrone and estradiol. Monitoring androstenedione levels aids in the diagnosis and therapeutic monitoring of conditions such as congenital adrenal hyperplasia (CAH) and polycystic ovary syndrome (PCOS). In CAH, there is an increased production of adrenal steroids due to enzyme deficiencies, leading to elevated androstenedione levels. Elevated androstenedione also assists in diagnosing PCOS, associated with hyperandrogenism, menstrual irregularities, and increased risk of diabetes and cardiovascular disease.
Special Instructions
To ensure consistency in test results, specimens should be collected at a consistent time before the administration of glucocorticoid doses, especially in patients undergoing treatment for conditions like CAH.
Limitations
The test was developed by LabCorp and has not been cleared or approved by the FDA. Random serum steroid levels in patients may fluctuate with time of day and glucocorticoid administration timing. Gel-barrier tubes are not recommended due to potential absorption issues affecting androstenedione levels.
Methodology
Mass Spectrometry
Biomarkers
LOINC Codes
- 1854-9
- 1854-9
Result Turnaround Time
4-6 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.5 mL
Container
Red-top tube. Do not use a gel-barrier tube.
Collection Instructions
Transfer separated serum to a plastic transport tube.
Storage Instructions
Refrigerate.
Causes for Rejection
Gross hemolysis; lipemia; gel-barrier tube
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |