Bacterial Vaginosis, Sialidase Activity
Also known as: BV (Sialidase) Activity, Vaginitis, Vaginosis
Use
Enzyme activity test for use in the detection of vaginal fluid specimens for sialidase activity, an enzyme produced by bacteria associated with bacterial vaginitis, including Bacteroides spp, Prevotella spp, and Mobiluncus spp and some G vaginalis. In a recent study, Bradshaw et al demonstrated the test to be 88% sensitive and 95% specific.
Special Instructions
Collecting specimens from the cervix should be avoided to reduce risk for obstetric patients and because cervical sialidase activity is typically higher than in vaginal samples. Specimens should not be collected from patients who have used vaginal creams, ointments, douches, spermicides, vaginal lubricants, or feminine sprays within 72 hours prior to testing. Immediate placement of the swabs into the transport tube is essential after sample collection.
Limitations
The test may give false-negative results if the specimen is insufficient or taken from patients undergoing antimicrobial therapy. Collecting specimens from inappropriate locations such as the cervix, or after the use of vaginal products, can also compromise the accuracy of the test results.
Methodology
Other
Biomarkers
LOINC Codes
- 41477-1
- 41477-1
Result Turnaround Time
2-3 days
Related Documents
For more information, please review the documents below
Specimen
Vaginal Swab
Volume
Swab
Minimum Volume
Not provided
Container
Copan double swab in Amies liquid medium
Collection Instructions
Using transport swabs provided, obtain a sample by contacting the lower one-third of the vaginal wall, rotating 10 to 20 seconds to absorb fluid. Immediately place the swabs into the transport tube.
Patient Preparation
Do not collect specimens from patients who have used a vaginal cream or ointment product, douched, or used spermicides, vaginal lubricants, or feminine sprays within 72 hours of testing.
Storage Instructions
Maintain specimen at room temperature for up to 48 hours.
Causes for Rejection
Specimen collected or transported other than previously described
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |