Bacterial Vaginosis (Sialidase) and Vaginal Yeast Culture
Also known as: BV (Sialidase) and Vaginal Yeast (Culture)
Use
BV (sialidase) activity: Enzyme activity test for use in the detection of vaginal fluid specimens for sialidase activity, an enzyme produced by bacteria associated with bacterial vaginitis, including Bacteroides spp, Prevotella spp, and Mobiluncus spp, and some G vaginalis. In a recent study, Bradshaw et al demonstrated the test to be 88% sensitive and 95% specific. Vaginal yeast culture: Detect yeast in vaginal fluid.
Special Instructions
It is important to avoid collecting specimens from the cervix as this might increase risk to obstetric patients, and cervical sialidase activity is usually higher than vaginal. Specimens should not be collected from patients who have used vaginal cream, ointment, douches, spermicides, vaginal lubricants, or feminine sprays within 72 hours of testing, as these factors may lead to false-negative results.
Limitations
If the sample is insufficient or collected from a patient undergoing antimicrobial therapy, the test may yield a false-negative result. Furthermore, inappropriate specimen collection or improper transport can lead to test rejection.
Methodology
Culture-based
Biomarkers
LOINC Codes
- 41477-1
- 41477-1
- 18483-8
Result Turnaround Time
4-5 days
Related Documents
For more information, please review the documents below
Specimen
Vaginal Swab
Volume
Swab
Minimum Volume
Not provided
Container
Copan double swab in Amies liquid medium
Collection Instructions
Using transport swabs provided, collect vaginal fluid sample by contacting the lower one-third of the vaginal wall, rotating for 10 to 20 seconds to absorb fluid. Immediately place the swabs into the transport tubes.
Storage Instructions
Maintain specimen at room temperature for up to 48 hours.
Causes for Rejection
Specimen collected or transport method other than described
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |