Enterohemorrhagic E coli (EHEC) Shiga Toxin, EIA
Also known as: E coli O157, EHEC, Stool Culture, Shiga Toxin
Use
Detect the presence of Shiga-toxin-producing enterohemorrhagic E coli
Special Instructions
For collection, a stool sample or a rectal swab should be placed in a stool culture transport vial like Para-Pak® orange. The specimen must be stored at room temperature and should not be frozen or overfilled in the transport media.
Limitations
This test specifically detects enterohemorrhagic E coli, particularly O157:H7 strains known for producing Shiga toxins. It does not detect other types of pathogenic E coli, such as enterotoxigenic (ETEC), enteropathogenic (EPEC), or enteroinvasive (EIEC). As a result, the test is not comprehensive for all forms of diarrheagenic E coli infections. A negative result does not rule out an E coli infection not related to Shiga toxin-producing strains, or other bacterial, viral, or parasitic infections causing similar symptoms.
Methodology
Immunoassay (EIA)
Biomarkers
LOINC Codes
- 21262-1
- 21262-1
Result Turnaround Time
2-3 days
Related Documents
For more information, please review the documents below
Specimen
Stool
Volume
1 to 2 g, 1 to 2 mL, or one swab
Minimum Volume
Rectal swab with obvious stool
Container
Stool culture transport vial (Para-Pak® orange)
Collection Instructions
Stool or rectal swab should be placed in stool culture transport vial. Culture collection swab may be used to collect rectal swabs or a swab of fecal material, then swab should be placed in stool culture transport vial.
Storage Instructions
Maintain specimen at room temperature.
Causes for Rejection
Specimen received in grossly leaking transport container; diapers; dry specimen; specimen submitted in fixative or additive; specimen received in expired transport media or incorrect transport device; inappropriate specimen transport conditions (not in a C&S vial or in an overfilled C&S vial); specimen received after prolonged delay in transport (usually more than 72 hours); specimen stored or transported frozen; wooden shaft swab in transport device; unlabeled specimen or name discrepancy between specimen and request label