Follicle-stimulating Hormone (FSH)
Also known as: Pituitary Gonadotropin
Use
Excessive FSH and LH are found in hypogonadism, anorchia, gonadal failure,1 complete testicular feminization syndrome, menopause, Klinefelter syndrome, alcoholism, and castration. FSH and LH are pituitary products, useful to distinguish primary gonadal failure from secondary (hypothalamic/pituitary) causes of gonadal failure, menstrual disturbances, and amenorrhea. Useful in defining menstrual cycle phases in infertility evaluation of women and testicular dysfunction in men. FSH is commonly used with LH, which also is a gonadotropin. Both are low in pituitary or hypothalamic failure. FSH and LH levels are high following menopause.
Special Instructions
This test may be affected by biotin supplementation. Patients should cease taking biotin at least 72 hours before sample collection to avoid interference with test results. It is advisable to inquire about biotin consumption from individuals indicated for this test.
Limitations
FSH secretion is pulsatile and varies in females during the menstrual cycle, complicating interpretation from single measurements. It is recommended to collect samples at intervals and pool them to mitigate pulsatile release effects. Additionally, mouse monoclonal antibodies in the assay can produce erroneous results in patients treated with such antibodies. Rarely, high levels of antibodies to streptavidin and ruthenium may interfere with the test, though additives in the assay minimize this risk.
Methodology
Immunoassay (ECLIA)
Biomarkers
LOINC Codes
- 15067-2
- 15067-2
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.7 mL
Container
Red-top tube, gel-barrier tube
Collection Instructions
If a red-top tube or plasma is used, transfer separated serum or plasma to a plastic transport tube.
Patient Preparation
Cease biotin supplementation at least 72 hours before sample collection.
Storage Instructions
Room temperature up to 14 days, refrigerated up to 14 days, frozen up to 14 days, stable for 3 freeze/thaw cycles.
Causes for Rejection
Use of oxalate, EDTA, or citrate plasma will result in rejection.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |