Hepatitis C Virus (HCV), Quantitative, RNA PCR (Nongraphical) With Reflex to Genotyping
Also known as: HCV Real-time PCR With Reflex to Genotype, HCV RNA Quantitation With Reflex to Genotype
Use
Quantitation of hepatitis C virus with reflex to HCV genotype if viral load is >1000 IU/mL
Special Instructions
Not provided.
Limitations
The test quantifies HCV RNA over the range of 15 IU/mL to 100,000,000 IU/mL. It may not accurately quantify levels outside this range. Caution is advised for low viral loads (<1000 IU/mL), especially as the assay may have specific performance limitations at such levels. Additional reflex tests may be required, which could impact turnaround time. Samples must be handled correctly to prevent rejection due to improper anticoagulants or failure to centrifuge plasma preparation tubes.
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 11011-4 - HCV RNA SerPl NAA+probe-aCnc
- 11011-4 - HCV RNA SerPl NAA+probe-aCnc
- 38180-6 - HCV RNA SerPl NAA+probe-Log IU
- 77202-0 - Laboratory comment Report
- 92731-9 - HCV Gentyp SerPl Seq
Result Turnaround Time
3-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
3 mL
Minimum Volume
1 mL
Container
Gel-barrier tube, lavender-top (EDTA) tube or plasma preparation tube (PPT™)
Collection Instructions
Centrifuge sample within 24 hours of collection. Transfer serum/plasma to a screw-cap polypropylene transport tube. Ship frozen (preferred). Plasma from a PPT™ can be frozen and shipped in situ.
Storage Instructions
Freeze (preferred) or refrigerate.
Causes for Rejection
Incorrect anticoagulant; PPT™ not centrifuged
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 72 hours |
| Frozen | 6 weeks |
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