Immunofixation (IFE), Serum, Protein Electrophoresis (PE), Serum, and Quantitative Free κ and λ Light Chains (FLC) Plus Ratio, Serum
Also known as: Free Light Chains, Quantitative, Serum, Freelite®, Globulins, Serum, IEP, Serum, IFE, Serum and Protein Electrophoresis, Serum
Use
Detect and identify monoclonal gammopathy (M protein) found on serum protein electrophoresis; diagnose myeloma, macroglobulinemia of Waldenström; evaluate amyloidosis; evaluate lymphoproliferative disease (malignant lymphoma and others) and collagen disease in general; diagnose and characterize immune deficient and dysgammaglobulinemic states
Special Instructions
State patient's age and clinical diagnosis on the test request form.
Limitations
This test requires accurate specimen handling to prevent hemolysis and is not suitable for plasma samples. It should be used in conjunction with other diagnostic techniques and guidelines, such as the NCCN Clinical Practice Guidelines in Oncology for Multiple Myeloma.
Methodology
Other
Biomarkers
LOINC Codes
- 55295-0
- 2465-3
- 2458-8
- 2472-9
- 2885-2
- 2862-1
- 2865-4
- 2868-8
- 2871-2
- 2874-6
- 33358-3
- 2336-6
- 1759-0
- 13169-8
- 77202-0
- 11502-2
- 36916-5
- 33944-0
- 48378-4
Result Turnaround Time
3-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
5.5 mL
Minimum Volume
2.2 mL
Container
Red-top tube or gel-barrier tube
Collection Instructions
Sample should be allowed to clot and the serum separated as soon as possible to prevent hemolysis.
Patient Preparation
Overnight fasting is preferred but not required.
Storage Instructions
IFE: Refrigerated. PE and FLC: Room temperature.
Causes for Rejection
Plasma sample
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |