Immunoglobulin G (IgG) Heavy and Light Chain (HLC) Pairs, κ and λ With Ratio
Also known as: Hevylite IgG, HLC IgG, IgG κ:λ HLC Ratio
Use
For the quantitative measurement of human IgG heavy chain and light chain intact immunoglobulin in serum. The IgG HLC ratio can be used when monitoring previously diagnosed IgG multiple myeloma in conjunction with other laboratory tests and clinical evaluations.
Special Instructions
Testing schedules may vary, and in some cases, additional time should be allowed for confirmatory or reflex tests.
Limitations
Decisions on patient evaluation and management must not be based solely on IgG κ, IgG λ, or IgG κ:IgG λ ratio measurements. Clinical history and other laboratory findings must be considered. Heavy and light chain quantitation should complement serum protein electrophoresis. The effect of therapeutic drugs on the measurement of IgG κ and IgG λ by this assay has not been evaluated. Small increases in monoclonal IgG proteins may not alter the HLC pair ratio. Initial detection of monoclonal proteins should include Immunofixation and Serum Protein Electrophoresis.
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
LOINC Codes
- 74862-4
- 74863-2
- 74868-1
Result Turnaround Time
4-6 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.75 mL
Minimum Volume
0.5 mL
Container
Red-top tube or gel-barrier tube
Collection Instructions
Separate serum immediately after coagulation (30 minutes) to prevent hemolysis.
Storage Instructions
Refrigerate.
Causes for Rejection
Microbially-contaminated specimen; specimen containing particulate matter; lipemic or hemolyzed specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |