Integrated 2
Also known as: AFP, Maternal, Down Syndrome
Use
Screening test for open neural tube defects, Down syndrome, and trisomy 18
Special Instructions
This test requires a preliminary specimen collected during the first trimester for the Integrated 1 test. Gestational age is calculated based on the crown-rump length provided with this initial specimen. For detailed patient information, the Maternal Prenatal Screening requisition form (0900) should be used. Additionally, it is necessary to ensure that maternal serum samples are obtained prior to amniocentesis to prevent fetal blood contamination.
Limitations
The test is dependent on two separate specimens: one from the first trimester and another from the second trimester. As a screening test, a positive result does not confirm a condition but suggests the possibility of neural tube defects or chromosomal abnormalities, prompting further diagnostic testing. Its development and performance characteristics were established by Labcorp; however, it does not have FDA clearance or approval.
Methodology
Immunoassay (CLIA)
Biomarkers
LOINC Codes
- 49586-1
- 11957-8
- 34970-4
- 49089-6
- 33882-2
- 18185-9
- 29463-7
- 43993-5
- 21484-1
- 44877-9
- 11878-6
- 12146-7
- 49035-9
- 48407-1
- 76348-2
- 1834-1
- 23811-3
- 19080-1
- 32166-1
- 2250-9
- 21264-7
- 23883-2
- 36904-1
- 48803-1
- 43995-0
- 49090-4
- 43994-3
- 47223-3
- 77202-0
- 11502-2
Result Turnaround Time
2-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
5 mL
Minimum Volume
3 mL
Container
Gel-barrier tube
Collection Instructions
Collect in serum separator tube with gel barrier. Allow blood to clot, avoiding hemolysis. Separate serum from cells by centrifugation. Transport spun tube to testing laboratory. Pour-off is not advised. Maternal serum specimens must be drawn prior to amniocentesis to avoid contamination with fetal blood.
Storage Instructions
Room temperature
Causes for Rejection
Gross hemolysis; gross lipemia; quantity not sufficient for analysis; improper specimen type
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 14 days |
| Frozen | 14 days |