Protein Electrophoresis, Serum With Reflex to IFE, Serum and Free κ and λ Light Chains, Serum

Casandra

Casandra Test Code LC37278Version 1 (DRAFT)

Performing Lab

Lab LabcorpLab Test ID 123041CPT Codes 84155, 84165

Protein Electrophoresis, Serum With Reflex to IFE, Serum and Free κ and λ Light Chains, Serum

Also known as: Free Light Chains, Quantitative, Serum, Freelite®, Globulins, Serum, IEP, Serum, IFE, Serum and Protein Electrophoresis, Serum

Clinical Use

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Use

Detect and identify monoclonal immunoglobulin gammopathies. Diagnose myeloma, macroglobulinemia of Waldenström. Immunofixation is used to evaluate monoclonal gammopathy (M protein) found on serum protein electrophoresis; evaluate amyloidosis. Has application to the evaluation of lymphoproliferative disease (malignant lymphoma and others) and collagen disease in general. Of use in the diagnosis and characterization of immune deficient and dysgammaglobulinemic states. The NCCN Clinical Practice Guidelines in Oncology (NCCN Guidelines™) recommend the use of serum free light chain assays for use in the initial diagnostic workup of multiple myeloma and related disorders. "Use of free light chain (FLC) assay along with SPEP and SIFE yields high sensitivity while screening for MM and related plasma cell disorders. Therefore, this assay is now included as a part of the initial diagnostic workup in the NCCN Multiple Myeloma Guidelines."1

Special Instructions

Not provided.

Limitations

The test may not detect all monoclonal gammopathies and can be affected by high levels of bilirubin or triglycerides, potentially leading to false negative results. Lipemia and hemolysis can cause specimen rejection. The test focus is on detecting monoclonal protein but may not identify complex beta-gamma bridging or subtle albumin anomalies.

Test Details