Syphilis: RPR With Reflex to RPR Titer and Treponemal Antibodies, Traditional Screening and Diagnosis Algorithm
Also known as: Nontreponemal Test, Serological Tests for Syphilis (STS), Syphilis Serology
Use
This test is intended to support screening and diagnosis of syphilis infections. This test aligns with the CDC-supported traditional serologic testing algorithm for syphilis using a combination of both treponemal and nontreponemal antibody tests. The traditional syphilis testing algorithm begins with the RPR (nontreponemal) test with positive samples reflexing to RPR titer and treponemal antibody testing. Interpretation of results obtained must take into account patient symptoms and clinical history.
Special Instructions
Not provided.
Limitations
Biological false positive results (RPR-reactive, Treponemal antibody-negative) can occur with infections other than syphilis, recent immunizations, autoimmune disorders, pregnancy, and injection drug use. These limitations need to be considered when interpreting the results. If recent exposure is suspected, submitting a new sample for testing in 2-4 weeks is recommended to ensure accuracy.
Methodology
Immunoassay (Chemiluminescence Immunoassay)
Biomarkers
LOINC Codes
- 20507-0 - RPR Ser Ql
- 20507-0 - RPR Ser Ql
- 31147-2 - RPR Ser-Titr
- 47236-5 - T palldm IgG+IgM SerPl Ql IA
- 93479-4 - Observation Imp
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
2 mL
Minimum Volume
1 mL
Container
Red-top tube or gel-barrier tube
Storage Instructions
Maintain specimen at room temperature.
Causes for Rejection
Grossly hemolyzed; grossly lipemic; gross bacterial contamination; plasma specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |
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