Syphilis: Treponemal Antibodies With Reflex to RPR and RPR Titer, Reverse Screening and Diagnosis Algorithm
Also known as: Syphilis, Trep-Sure™, Treponemal
Use
This test is intended to support screening and diagnosis of syphilis infections. This test aligns with the CDC-supported reverse serologic testing algorithm for syphilis using a combination of both treponemal and nontreponemal antibody tests. The reverse syphilis testing algorithm begins with a treponemal antibody assay. Positive samples reflex to RPR (nontreponemal). If the RPR is positive, RPR titer will be determined. If the RPR is negative, a second treponemal antibody assay will be performed to confirm the presence of treponemal antibodies. Interpretation of results obtained must take into account patient symptoms and clinical history.
Special Instructions
Not provided.
Limitations
Treponemal antibodies tend to persist for life despite treatment, making this assay unsuitable for monitoring response to treatment or assessing treatment outcomes. False-positive results can occur in individuals with non-syphilis treponemal infections like bejel, pinta, and yaws.
Methodology
Immunoassay (CLIA)
Biomarkers
RPR
AnalyteTreponemal Antibodies, TPPA
ProteinTreponemal antibody
Protein
LOINC Codes
- 47236-5 - T palldm IgG+IgM SerPl Ql IA
- 47236-5 - T palldm IgG+IgM SerPl Ql IA
- 20507-0 - RPR Ser Ql
- 31147-2 - RPR Ser-Titr
- 24312-1 - T palldm Ab Ser Ql Aggl
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.5 mL
Container
Red-top tube or gel-barrier tube
Storage Instructions
Room temperature
Causes for Rejection
Grossly hemolyzed; grossly lipemic; gross bacterial contamination; plasma specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |
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