B-cell Receptor IGH Gene Rearrangement, PCR
Also known as: Heavy Chain Gene Rearrangement, Immunoglobulin Heavy Chain, B-Cell Clonality, B cell PCR
Use
This test detects clonal immunoglobulin heavy chain (IGH) gene rearrangements by PCR and fragment analysis to assess B‑cell clonality in suspected lymphoproliferative disorders such as leukemia or lymphoma, and provides a pathologist‑integrated interpretation. Useful in establishing clonality, particularly in B‑cell neoplasms. ([questdiagnostics.com](https://www.questdiagnostics.com/healthcare-professionals/clinical-education-center/faq/faq124?utm_source=openai))
Special Instructions
Not provided.
Limitations
IGH PCR may yield false‑negative results in up to 5‑20% of B‑cell neoplasms due to absent or incomplete rearrangements (e.g., immature B‑cell neoplasms) or extensive somatic hypermutation (e.g., follicular lymphoma). For increased sensitivity, combined IGH and IGK testing (B‑cell clonality panel) is recommended in such contexts. ([questdiagnostics.com](https://www.questdiagnostics.com/healthcare-professionals/clinical-education-center/faq/faq124?utm_source=openai))
Methodology
PCR-based (PCR)
Biomarkers
IGH
Gene
Result Turnaround Time
5 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
3‑5 mL
Minimum Volume
3 mL
Container
Lavender (EDTA)
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