Lyme Disease Ab with Reflex to Blot (IgG, IgM)

Casandra

Casandra Test Code QD93287Version 1 (DRAFT)

Performing Lab

Lab Quest DiagnosticsLab Test ID 6646CPT Codes 86617, 86618

Lyme Disease Ab with Reflex to Blot (IgG, IgM)

Also known as: Borrelia burgdorferi

Clinical Use

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Use

This test follows the CDC‑recommended standard two‑tier testing algorithm (STTT) for Lyme disease. It begins with an enzyme immunoassay (EIA) or immunofluorescence assay (IFA) to screen for antibodies to Borrelia burgdorferi; if the result is positive or equivocal, a confirmatory immunoblot (Western blot) for both IgG and IgM antibodies is performed.

Special Instructions

Reflex testing — if the Lyme Disease Antibody screen is ≥0.90 (or positive/equivocal), the test will reflex to immunoblot for IgG and IgM (TC: 8593) at additional charge. Orderable separately as well.

Limitations

Antibodies may not become detectable until weeks after infection; early infections may yield false‑negative results. Positive IgM results beyond 30 days of symptom onset may be less reliable. Persistent antibodies may remain post‑infection and cannot distinguish active from prior infection. Reflex immunoblot is required for confirmation; screening alone is insufficient for diagnosis.

Test Details