Nicotine and Cotinine, Serum/Plasma
Use
This assay is used for the detection of nicotine and cotinine in serum and plasma to determine the tobacco exposure status of the individual. Nicotine has a short half‑life of approximately forty minutes; its presence may indicate recent tobacco exposure. Cotinine, the major nicotine metabolite, has a half‑life of 24 hours and is detectable for several days after cessation of tobacco exposure.
Special Instructions
Not provided.
Limitations
Not provided.
Methodology
Mass Spectrometry
Biomarkers
Cotinine
AnalyteNicotine
Analyte
Result Turnaround Time
3-4 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL serum collected in a red‑top tube (no gel)
Minimum Volume
0.5 mL serum or plasma • 2 mL whole blood
Container
Plastic, screw‑cap vial
Collection Instructions
Spin and transfer serum or plasma into a plastic, screw‑capped vial; also acceptable: plasma collected in EDTA (lavender‑top) or royal blue‑top tube; transport EDTA whole blood
Storage Instructions
Refrigerated (cold packs)
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | 21 days |
| Frozen | 30 days |
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