Adrenocorticotropic Hormone Stimulation, 60 Minutes
Also known as: CORTISOL60
Use
This test is used to screen, diagnose, and monitor diseases associated with excess or deficient cortisol production. It is particularly useful for assessing adrenal insufficiency and hypopituitarism. The test evaluates the adrenal glands' response to adrenocorticotropic hormone (ACTH) by measuring cortisol levels 60 minutes after administration of cosyntropin.
Special Instructions
Specimen collection requires strict adherence to timing; collect 1 mL of serum 60 minutes after cosyntropin injection, ensuring proper labeling with the time of draw. Maintain specimen stability by separating serum from cells promptly and refrigerating for up to 4 days. Avoid repeated freeze/thaw cycles when storing frozen.
Limitations
The accuracy of this test may be affected by improper specimen collection, delayed processing, or inappropriate storage conditions. Hemolysis or lipemia may interfere with the assay results. The test may not accurately reflect adrenal function in patients receiving glucocorticoid therapy or with certain medical conditions. Results must be interpreted in conjunction with clinical findings.
FDA ApprovedNew York Approved
Methodology
Immunoassay (ECLIA)
Biomarkers
LOINC Codes
- 26528-0
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.4 mL
Container
Serum separator tube (SST) or Plain Red, Green (lithium heparin), Lavender (K2 EDTA and K3 EDTA), or Pink (K2 EDTA)
Collection Instructions
Collect 1 timed specimen at 60 minutes after administration of 250 micrograms of cosyntropin IV.
Storage Instructions
Refrigerated for up to 4 days. Frozen for up to 12 months. Avoid repeated freeze/thaw cycles.
Causes for Rejection
Improper timing or labeling, hemolysis, lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 4 days |
| Frozen | 12 months |
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