Anti-Centromere Ab by IFA (RDL)
Also known as: Centromere
Use
The most common setting for finding anti-centromere antibodies is in patients with a limited cutaneous form of scleroderma. Anti-centromere abs are found in 22% of patients with systemic sclerosis and in 12% of patients with primary biliary cirrhosis, and are rarely present in normal individuals.
Special Instructions
This test is a component of ANA Profile 11, ANA 12 Profile, ANA 12 Plus Profile, Scleroderma Comprehensive Profile, and Scleroderma Comprehensive Profile Plus. Separate serum from cells within one hour of collection. Transfer to a plastic transport tube before shipping. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Limitations
Anti-centromere antibodies are found in 46% of patients with Limited Systemic Sclerosis and 11% in Diffuse Systemic Sclerosis. The accuracy of this test may be influenced by sample integrity; grossly hemolyzed, bacterial contamination, lipemic, icteric, and non-serum specimen types are causes for rejection. The sample stability is contingent on proper storage, with room temperature stability for 7 days, refrigerated for 14 days, and frozen for 60 days, stable through one freeze/thaw cycle.
Methodology
Immunoassay (Indirect fluorescent antibody (IFA))
Biomarkers
LOINC Codes
- 5077-3
- 5077-3
Result Turnaround Time
14-21 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.5 mL
Container
Red-top tube or gel-barrier tube
Collection Instructions
Separate serum from cells within one hour of collection. Transfer to a plastic transport tube before shipping.
Storage Instructions
Refrigerate or freeze.
Causes for Rejection
Grossly hemolyzed; bacterial contamination; lipemic specimen; icteric specimen; non-serum specimen types
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 14 days |
| Frozen | 60 days |
Other tests from different labs that may be relevant