Chimerism, Posttransplant, Sorted Cells (CD34+ Cells)
Also known as: STRPOST-34
Use
This test is used to monitor the engraftment of donor cells following allogeneic stem cell transplant. It compares posttransplant genotypes to pretransplant recipient and donor genotypes to assess the presence and percentage of donor cells in the patient's blood. Monitoring chimerism levels is crucial to the management and prognosis of transplant recipients.
Special Instructions
Each posttransplant specimen and test ordered must have a unique ARUP accession number. It is essential to provide the results and date of the patient's most recent white blood cell count and differential for proper assessment. For bone marrow specimens submitted for cell sorting, information about the general cellularity of the patient's bone marrow is required.
Limitations
Diagnostic errors can occur due to rare sequence variations. The limit of detection for minor cell populations is set at 2 percent. Proper interpretation requires correlation with the clinical status and consideration of the time since transplantation. This test has not been cleared or approved by the U.S. Food and Drug Administration and is performed as a laboratory-developed test under CLIA regulations.
New York Approved
Methodology
PCR-based
Biomarkers
Result Turnaround Time
5-12 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
2 mL
Minimum Volume
2 mL
Container
Lavender (EDTA), pink (K2EDTA), or yellow (ACD solution A or B)
Storage Instructions
Ship overnight. Specimens should be received within 24 hours of collection for optimal isolation of the requested cell line(s).
Causes for Rejection
Clotted or hemolyzed specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | 72 hours |
| Frozen | Unacceptable |
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