Myeloproliferative Disorders Panel by FISH
Also known as: FISH MPD P
Use
This test is used in the limited workup of myeloproliferative neoplasms when other optimal cytogenetic studies have been conducted. It helps in excluding cryptic BCR-ABL1 rearrangement in chronic myelogenous leukemia and excludes PDGFRA abnormalities in cases of neoplastic eosinophilia. The panel includes probes to detect various clinically significant genetic rearrangements associated with these disorders.
Special Instructions
The test must be ordered using the Oncology test request form #43099 or through the ARUP interface. A processing fee will be charged if the procedure is canceled after the test setup or if the specimen is not adequately prepared for culture. A Bone Marrow/PBL Culture Processing Fee will be added if chromosome analysis is not performed at ARUP on the same sample.
Limitations
The test is performed using fluorescence in situ hybridization (FISH), which may have limitations in detecting low-level mosaicism, or mutations not involving the specified probe regions. Such limitations should be considered in conjunction with clinical findings and other diagnostic tests. Paraffin-embedded, clotted, or frozen specimens are unacceptable, potentially limiting the sample suitability.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
3-9 days
Related Documents
For more information, please review the documents below
Specimen
Bone Marrow
Volume
3 mL
Minimum Volume
1 mL
Container
Green (sodium heparin) tube or heparinized syringe
Collection Instructions
Nondiluted bone marrow should be collected in a heparinized syringe. It should be transferred to a green (sodium heparin) tube.
Causes for Rejection
Frozen specimens, paraffin-embedded specimens, clotted specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 48 hours |
| Frozen | Unacceptable |
Other tests from different labs that may be relevant