Alpha-1-Antitrypsin (AAT) by Immunohistochemistry
Also known as: A1 AT IHC
Use
Aids in histologic diagnosis of Alpha‑1‑Antitrypsin deficiency. This immunohistochemical stain supports the evaluation of tissue morphology to detect AAT accumulation, serving as an adjunct to confirm alpha‑1‑antitrypsin deficiency in relevant clinical contexts.
Special Instructions
This test is performed as a stain-and-return (technical) service only. Submit by electronic request or by using ARUP Immunohistochemistry Stain Form (#32978) with an ARUP client number. ARUP supply #47808 transport kit recommended for tissue blocks or slides.
Limitations
Unacceptable specimens include non‑representative tissue types or depleted specimens. Precut slides should not be oven-baked. The service is technical only, meaning interpretation occurs elsewhere.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 94736-6
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
(Min: 2 slides)
Container
tissue block or 5 unstained positively charged slides (3‑ to 5‑micron thick)
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen (cells must be prepared into a cellblock). Protect paraffin block and/or slides from excessive heat. Transport tissue block or 5 unstained (3‑ to 5‑micron thick), positively charged slides in a tissue transport kit (ARUP supply #47808). If sending precut slides, do not oven bake.
Storage Instructions
Room temperature or refrigerated. Ship in cooled container during summer months.
Causes for Rejection
Specimens submitted with non‑representative tissue type. Depleted specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |