Beta‑Catenin‑1 by Immunohistochemistry
Also known as: BCAT1 IHC
Use
Immunohistochemical detection of beta‑catenin expression/localization in tissue or cell specimens is performed as a technical stain for research or diagnostic adjunct purposes. Aberrant cytoplasmic or nuclear localization, compared to normal membrane localization, may support evaluation of tumors with Wnt/APC pathway involvement. Interpretation must be performed separately by pathology if diagnostic interpretation is required.
Special Instructions
Not provided.
Limitations
Specimens must be representative tissue or cell block; non‑representative or depleted specimens are unacceptable. If sending precut slides, do not oven bake. Frozen specimens unacceptable. Interpretation is not included; pathologist consultation must be ordered separately. Controls required, interpretation subject to clinical context.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 21659-8 - CTNNB1 gene Mut Anl Bld/T
- 94736-6 - Tissue block ID Spec
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
5 unstained slides (min: 2 slides) or tissue block
Container
tissue transport kit (ARUP supply #47808)
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen (cells must be prepared into a cellblock). Protect paraffin block and/or slides from excessive heat. Transport tissue block or 5 unstained (3‑ to 5‑micron thick sections), positively charged slides in tissue transport kit.
Storage Instructions
Room temperature or refrigerated; ship in cooled container during summer months.
Causes for Rejection
Non‑representative tissue type; depleted specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |
Other tests from different labs that may be relevant