Bordetella pertussis Antibodies, IgA, IgG, and IgM by ELISA with Reflex to Immunoblot

Casandra

Casandra Test Code AR05393Version 1 (DRAFT)

Performing Lab

Lab ARUP LaboratoriesLab Test ID 2001775CPT Code 86615

Bordetella pertussis Antibodies, IgA, IgG, and IgM by ELISA with Reflex to Immunoblot

Also known as: BORDPAN3

Clinical Use

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Use

The Bordetella pertussis Antibodies, IgA, IgG and IgM test is intended for the detection of antibodies against Bordetella pertussis. It is used to assess immune response in patients suspected of having a recent or past infection with Bordetella pertussis. The presence of specific antibodies can indicate a current or recent exposure or a past immunization against B. pertussis. When a result is IgG or IgA positive, further immunoblot testing is performed to confirm the findings.

Special Instructions

Specimens must be labeled as acute or convalescent and with the appropriate collection dates. Convalescent specimens should be received within 30 days of acute specimen collection for optimal results. Stability varies depending on temperature: Ambient conditions allow for up to 48 hours post-separation, while refrigerated conditions allow for up to 2 weeks and frozen specimens are stable for up to a year; however, New York clients have different stability requirements.

Limitations

The IgM component may produce false positive results, thus treatment decisions should be based on the immunoblot results rather than the ELISA for IgM antibodies. Additional testing with immunoblots for IgA and IgG is performed if initial ELISA results exceed certain thresholds, indicating higher antibody levels. This test, as a serology method, may be less effective for assessing acute infections compared to PCR or culture.

Test Details