Bordetella pertussis Antibodies, IgA, IgG, and IgM by ELISA with Reflex to Immunoblot
Also known as: BORDPAN3
Use
The Bordetella pertussis Antibodies, IgA, IgG and IgM test is intended for the detection of antibodies against Bordetella pertussis. It is used to assess immune response in patients suspected of having a recent or past infection with Bordetella pertussis. The presence of specific antibodies can indicate a current or recent exposure or a past immunization against B. pertussis. When a result is IgG or IgA positive, further immunoblot testing is performed to confirm the findings.
Special Instructions
Not provided.
Limitations
The IgM component may produce false positive results, thus treatment decisions should be based on the immunoblot results rather than the ELISA for IgM antibodies. Additional testing with immunoblots for IgA and IgG is performed if initial ELISA results exceed certain thresholds, indicating higher antibody levels. This test, as a serology method, may be less effective for assessing acute infections compared to PCR or culture.
Methodology
Immunoassay (ELISA)
Biomarkers
IgA
AntibodyIgG
AntibodyIgM
Antibody
LOINC Codes
- 42328-5 - B pert IgA Ser IA-aCnc
- 42329-3 - B pert IgM Ser IA-aCnc
- 42330-1 - B pert IgG Ser IA-aCnc
- 42328-5 - B pert IgA Ser IA-aCnc
- 42330-1 - B pert IgG Ser IA-aCnc
- 42329-3 - B pert IgM Ser IA-aCnc
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.3 mL
Container
ARUP Standard Transport Tube
Collection Instructions
Collect using Serum Separator Tube. Separate serum from cells within 2 hours of collection.
Causes for Rejection
Bacterially contaminated, heat-inactivated, hemolyzed, icteric, lipemic, or turbid specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 2 weeks |
| Frozen | 1 year |
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