Brachyury by Immunohistochemistry
Also known as: BRACHY IHC
Use
This test employs immunohistochemistry staining for brachyury—a transcription factor used diagnostically, particularly in differentiating chordoma from other tumor types. It is intended for clinical purposes and performed in a CLIA-certified laboratory, aiding pathologic interpretation through stain-and-return technical service. It was developed and its performance characteristics determined by ARUP Laboratories. ([ltd.aruplab.com](https://ltd.aruplab.com/Tests/Pub/3003824?utm_source=openai))
Special Instructions
Not provided.
Limitations
This is a laboratory-developed test (LDT) and has not been cleared or approved by the US Food and Drug Administration. Interpretations must consider technical limitations inherent to immunohistochemistry and stain return service. ([ltd.aruplab.com](https://ltd.aruplab.com/Tests/Pub/3003824?utm_source=openai))
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 94736-6 - Tissue block ID Spec
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen (cells must be prepared into a cellblock). Transport tissue block or 5 unstained (3- to 5-micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808). (Min: 2 slides). If sending precut slides, do not oven bake.
Storage Instructions
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.
Causes for Rejection
Specimens submitted with non-representative tissue type. Depleted specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |
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