Cathepsin K by Immunohistochemistry
Also known as: CATHK IHC
Use
Cathepsin K by Immunohistochemistry is used for the qualitative detection of Cathepsin K in tissue sections. This immunohistochemistry stain is often utilized in pathology to help differentiate types of cells in a tissue sample, particularly in the diagnosis of various diseases that involve tissue remodeling, such as osteoporosis, arthritis, fibrotic diseases, and certain types of cancer. By identifying the presence and patterns of Cathepsin K expression, pathologists are able to provide insights into the functional status of tissues and assist in clinical decision-making.
Special Instructions
To order this test, submit an electronic request. If electronic ordering is unavailable, use the ARUP Immunohistochemistry Stain Form with an ARUP client number. Utilize the Tissue Transport Kit (ARUP supply #47808) for optimal sample integrity. The test is performed as a stain and return service only, so ensure correct submission of required details and follow guidelines provided in the ARUP Immunohistochemistry Stain Offerings brochure.
Limitations
This test may have limitations regarding the variety of tissue samples and the quality of staining. Samples that are not perfectly preserved or processed may result in suboptimal staining, leading to difficulties in interpretation. The specificity and sensitivity of the test may also be influenced by factors such as tissue type, preparation method, and the inherent variability of immunohistochemical methods. The test is not to be used as the sole diagnostic tool but rather as a supplement to other diagnostic assays and clinical assessments.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
2 slides
Container
Tissue Transport Kit (ARUP supply #47808)
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen (cells must be prepared into a cellblock). Protect paraffin block and/or slides from excessive heat. Transport tissue block or 5 unstained (3- to 5-micron thick sections), positively charged slides.
Storage Instructions
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.
Causes for Rejection
Tissue not mounted on positively charged slides. Specimens submitted with nonrepresentative tissue type. Depleted specimens. Serum, blood, or bodily fluids.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |