Cathepsin K by Immunohistochemistry

Also known as: CATHK IHC

Clinical Use

Use

Cathepsin K by Immunohistochemistry is used for the qualitative detection of Cathepsin K in tissue sections. This immunohistochemistry stain is often utilized in pathology to help differentiate types of cells in a tissue sample, particularly in the diagnosis of various diseases that involve tissue remodeling, such as osteoporosis, arthritis, fibrotic diseases, and certain types of cancer. By identifying the presence and patterns of Cathepsin K expression, pathologists are able to provide insights into the functional status of tissues and assist in clinical decision-making.

Special Instructions

Not provided.

Limitations

This test may have limitations regarding the variety of tissue samples and the quality of staining. Samples that are not perfectly preserved or processed may result in suboptimal staining, leading to difficulties in interpretation. The specificity and sensitivity of the test may also be influenced by factors such as tissue type, preparation method, and the inherent variability of immunohistochemical methods. The test is not to be used as the sole diagnostic tool but rather as a supplement to other diagnostic assays and clinical assessments.

Test Details

New York Approved

Methodology

Immunoassay (IHC)

Biomarkers

Cathepsin K

Protein

IHC

Expression • Categorical (e.g., Positive / Negative / Indeterminate)

Result Turnaround Time

1-3 days

Specimen

Tissue (FFPE)

Volume

Not provided

Minimum Volume

2 slides

Container

Tissue Transport Kit (ARUP supply #47808)

Collection Instructions

Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen (cells must be prepared into a cellblock). Protect paraffin block and/or slides from excessive heat. Transport tissue block or 5 unstained (3- to 5-micron thick sections), positively charged slides.

Storage Instructions

Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.

Causes for Rejection

Tissue not mounted on positively charged slides. Specimens submitted with nonrepresentative tissue type. Depleted specimens. Serum, blood, or bodily fluids.