Smad4 by Immunohistochemistry
Also known as: SMAD4 IHC
Use
This test is used to identify the presence and extent of SMAD4 protein in tissue samples. SMAD4, also known as DPC4, is a tumor suppressor gene commonly mutated or deleted in pancreatic and colorectal carcinomas. Immunohistochemistry (IHC) staining is performed for pathological examination to assist in diagnosing and determining the prognosis of cancer. SMAD4 expression can provide insights into tumor progression and response to potential treatment protocols, making it an important tool in clinical decision-making.
Special Instructions
For immunohistochemistry orders, submit an electronic request or use the ARUP Immunohistochemistry Stain Form (#32978) with an ARUP client number if electronic ordering capability is not available. All specimens should be transported using the ARUP tissue transport kit (ARUP supply #47808), which can be ordered online through ARUP Connect™ or by contacting ARUP Client Services.
Limitations
This test is a laboratory developed test (LDT) and has not been cleared or approved by the FDA. It is intended for clinical purposes and its performance characteristics have been determined by ARUP Laboratories. As a stain and return (technical) service, it does not include interpretation of results. Specimens with non-representative tissue type or depleted specimens are unacceptable.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- 94736-6
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
2 slides
Container
Tissue transport kit (ARUP supply #47808)
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen. Protect paraffin block and/or slides from excessive heat. Transport tissue block or 5 unstained (3- to 5-micron thick sections), positively charged slides.
Storage Instructions
Room temperature. Refrigerated also acceptable. Ship in cooled container during summer months.
Causes for Rejection
Specimens submitted with non-representative tissue type. Depleted specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinite |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |