Bordetella pertussis and Bordetella parapertussis, Real-time DNA PCR
Also known as: B pertussis and B parapertussis, DNA PCR (Real-time), Whooping Cough
Use
This assay is intended to be used as an aid to the diagnosis of Bordetella pertussis (whooping cough) and Bordetella parapertussis (whooping cough-like syndrome).
Special Instructions
Not provided.
Limitations
The test may yield false-positive results for Bordetella pertussis DNA if Bordetella holmesii is present in the sample, as the target region, IS481, is shared by both organisms. Results must therefore be interpreted in conjunction with clinical findings and other laboratory tests to confirm the diagnosis. The test is not FDA cleared or approved, which may restrict its use in certain clinical situations.
Methodology
PCR-based (Real-time PCR)
Biomarkers
LOINC Codes
- 90441-7 - B pert+parapert DNA Pnl Nph
- 91815-1 - B pert DNA Upper resp Ql NAA+probe
- 91816-9 - B parapert DNA Upper resp Ql NAA+probe
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Nasopharyngeal Swab
Volume
One NP/throat swab
Minimum Volume
Not provided
Container
Flocked NP swab submitted dry in plastic transport sleeve (PeopleSoft N° 93307)
Storage Instructions
Maintain specimen at room temperature or refrigerate. Do not freeze charcoal transport swabs or dry swabs. Swabs in UTM and NP aspirates/washes may be frozen for seven days.
Causes for Rejection
Calcium alginate swabs; swabs in noncharcoal-containing transport media; swabs in Regan-Lowe culture media
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 5 days |
| Refrigerated | 7 days |
| Frozen | 7 days |
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