Connective Tissue Disease (CTD) Cascade
Also known as: Autoimmune (ANA) Rheumatic Disease
Use
This profile may be useful in diagnosing connective tissue or systemic autoimmune rheumatic disease.
Special Instructions
The test begins with Antinuclear Antibodies (ANA) by IFA. If ANA is positive, reflex tests are performed including Anti-DsDNA, Anti-Sm, Complement C3, Complement C4, Anti-Ro (SS-A), and more. If ANA by IFA is negative, RheumAssure is conducted, including tests like Rheumatoid Factor. Further reflexes are conducted based on these results, including Anti-TPO and Anti-Thyroglobulin testing.
Limitations
The CTD Cascade may not detect all forms of connective tissue diseases, as some may not produce specific antibodies detectable by the included tests. Results should be interpreted in conjunction with clinical findings, patient history, and other diagnostic procedures. Reflex testing may not proceed if sample volume is insufficient.
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 42254-3
- 42254-3
- 20398-4
- 20399-2
- 20401-6
- 53993-2
- 53003-0
- 54006-2
- 54155-7
- 53998-1
- 53006-3
- 77202-0
- 5077-3
- 47299-3
- 4485-9
- 4498-2
- 33569-5
- 45142-7
- 11090-8
- 57662-9
- 32580-3
- 26975-3
- 5348-8
- 35333-4
- 69048-7
- 3181-5
- 3182-3
- 21108-6
- 16136-4
- 11572-5
- 57093-7
- 75880-5
- 8099-4
- 56536-6
Result Turnaround Time
10 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
5 mL
Minimum Volume
4 mL
Container
2 red-top or gel-barrier tubes; transfer tubes
Collection Instructions
Separate serum from cells within one hour of collection. Transfer to a plastic transport tube before shipping.
Storage Instructions
Refrigerate.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 14 days |