Dihydrotestosterone (DHT), Free, LC/MS/Dialysis

Also known as: Free 5-α-dihydrotestoterone

Clinical Use

Use

Dihydrotestosterone measurement is used to diagnose 5α-reductase deficiency and for the evaluation of androgen utilization. Dihydrotestosterone is produced by the reduction of testosterone by 5α-reductase in the target organs. Circulating dihydrotestosterone is tightly bound to sex hormone-binding globulin. The free fraction represents the dihydrotestosterone available to act on tissues.

Special Instructions

Not provided.

Limitations

This test has been developed and its performance characteristics determined by LabCorp. However, it has not been cleared or approved by the US Food and Drug Administration (FDA), which may impact its acceptance for certain clinical applications. Incorrect sample handling, such as using EDTA plasma or samples that are grossly hemolyzed, icteric, or lipemic, can compromise the test results.

Test Details

Methodology

Mass Spectrometry

Biomarkers

Dihydrotestosterone (DHT)

Analyte

Dihydrotestosterone

Concentration / Level • Quantitative (Continuous) (e.g., copies/mL, % expression)

DHT, Percent Free Dialysis

Concentration / Level • Quantitative (Continuous) (e.g., copies/mL, % expression)

DHT, Free

Concentration / Level • Quantitative (Continuous) (e.g., copies/mL, % expression)

LOINC Codes

Result Codes

1848-1 - Androstanolone SerPl-mCnc
13882-6 - Androstanolone Free MFr SerPl
14047-5 - Androstanolone Free SerPl-mCnc

Result Turnaround Time

9-15 days

Specimen

Serum

Volume

3 mL

Minimum Volume

1.2 mL

Container

Red-top tube, gel-barrier tube, or green-top (heparin) tube

Collection Instructions

Separate serum or plasma from cells within four hours of collection. Send serum or plasma in a plastic transport tube. Transfer specimen to a plastic transport tube before freezing.