HER-2/neu IHC, Breast Paraffin
Also known as: c-erb-2
Use
The HER-2/neu protein is a product of the HER-2/neu oncogene, and the detection of overexpression of the HER-2/neu protein has been associated in clinical evaluations with a decreased survival time and a decreased time to relapse in patients with breast cancer.1-3 HER-2/neu overexpression is also a predictor of response to certain breast cancer therapies, including targeted immunotherapies.4-6
Special Instructions
Not provided.
Limitations
Use of fixatives other than 10% neutral-buffered formalin or fixation times less than six hours or more than 72 hours may not yield reliable results. Decalcification procedures may negatively affect antigenicity and DNA quality.
Methodology
Immunoassay (IHC)
Biomarkers
HER-2/neu
Protein
LOINC Codes
- 18474-7 - Her2 Ag Tiss Ql ImStn
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
One paraffin block or five unstained, positively-charged tissue slides cut at 4 to 5 microns and dried for one hour at 60°C
Minimum Volume
Not provided
Container
Paraffin block transport pouch or slide mailer
Collection Instructions
Specimen should be fixed in 10% neutral-buffered formalin. Time from tissue acquisition to fixation should be as short as possible. Fixation time should be between six and 72 hours according to ASCO/CAP guidelines.
Storage Instructions
Maintain specimen at room temperature.
Causes for Rejection
Tumor other than breast tumor
Other tests from different labs that may be relevant