HLA Class I Antibody, Panel-reactive Antibody (PRA)
Also known as: HLA Antibody, PRA, HLA Class 1 Antibody, White Cell Antibody
Use
Solid organ transplantation
Special Instructions
Not provided.
Limitations
The test detects antibodies of the IgG isotype; it does not detect IgM antibodies, which are often indicative of autoantibodies. This limitation means that the test focuses solely on IgG-mediated immune responses and may not fully characterize the antibody profile related to potential graft rejection. Furthermore, factors such as lipemic or hemolyzed specimens can cause interference, potentially leading to rejection of the sample. Despite these limitations, the test offers valuable insights into compatibility for organ transplantation.
Methodology
Immunoassay (Luminex)
Biomarkers
HLA Class I Antibody
Protein
LOINC Codes
- 46994-0 - HLA-A+B+C Ab NFr Ser
- 46994-0 - HLA-A+B+C Ab NFr Ser
- 49549-9 - Ref lab test method
Result Turnaround Time
3-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
7 mL
Minimum Volume
3 mL
Container
Red-top tube or gel-barrier tube
Collection Instructions
To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Storage Instructions
Maintain specimen at room temperature. Protect from extreme heat or cold. Serum may be frozen.
Causes for Rejection
Highly lipemic or hemolyzed specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 2 days |
| Refrigerated | 1 month |
| Frozen | 2 years |
Other tests from different labs that may be relevant