HLA Class II Antibody C1q Binding Assay
Also known as: C1q Fixing Class II Human Leukocyte Antigen Antibodies, Complement Fixing Antibody to Class II HLA Antigens
Use
The HLA Class II Antibody C1q Binding Assay is utilized to detect antibodies capable of binding C1q, which may indicate the presence of complement-fixing antibodies. These antibodies can play a role in transplant rejection by activating the complement system, potentially causing damage to the transplanted organ.
Special Instructions
It is important to submit separate frozen specimens for each test requested to prevent delays in turnaround time when ordering multiple tests on frozen samples. Ensure proper use of collection tubes to avoid causes for rejection.
Limitations
The test is limited by potential interferences that could arise from hemolysis and the use of incorrect collection tubes, both of which are grounds for specimen rejection. Additionally, the stability of the sample must be maintained by adhering to the specified storage and temperature conditions.
Methodology
Other
Biomarkers
LOINC Codes
- 46995-7
- 49549-9
Result Turnaround Time
3-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
7 mL
Minimum Volume
3 mL
Container
Red-top tube or gel-barrier tube
Collection Instructions
To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Patient Preparation
None
Storage Instructions
Refrigerate.
Causes for Rejection
Hemolysis; wrong collection tube
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | Up to 11 months |