Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Nongraphical) With Reflex to PhenoSense®
Use
This test is used to detect and quantitate human immunodeficiency virus type 1 (HIV-1) in plasma. It incorporates a reflex mechanism to perform phenotype testing (PhenoSense®) if the viral load exceeds 500 copies/mL, which helps in determining the virus's resistance to antiretroviral drugs. This quantitation is essential for managing and tailoring antiretroviral therapy for patients with HIV-1 infection.
Special Instructions
Ensure that specimens are collected in the specified tubes and processed within the recommended time frame to avoid delays. Plasma should be frozen and stored in screw-cap tubes, as 'pop-top' or 'snap-cap' tubes are unsuitable. Separate frozen samples are required for the PhenoSense® component.
Limitations
The assay has a dynamic range of 20 to 10,000,000 copies/mL, limiting its sensitivity for detecting viral loads below 20 copies/mL. Variability in sample processing or storage can affect the results, particularly if specimens are not appropriately frozen or are transferred in unsuitable containers. Use of green-top tubes or receiving insufficient samples may lead to rejection of specimens.
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 20447-9
- 20447-9
- 29541-0
- 45182-3
- 49665-3
- 48767-8
Result Turnaround Time
17-25 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
6 mL
Minimum Volume
4 mL
Container
Screw-cap polypropylene frozen transport tubes
Collection Instructions
Collect in three lavender-top (EDTA) tubes or three PPT™ tubes. Centrifuge within six hours and transfer plasma to screw-cap tubes before freezing. Avoid green-top (heparin) tubes and ensure samples are frozen for shipping.
Storage Instructions
Separate frozen sample is required for PhenoSense® testing. Plasma stable at room temperature (1 day) or refrigerated (5 days); freeze for long-term storage.
Causes for Rejection
Hemolysis; use of green-top tube; pop-top or snap-cap tubes; insufficient quantity; frozen PPT™
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 1 day |
| Refrigerated | 5 days |