Human Immunodeficiency Virus 1 (HIV-1) by Quantitative NAAT with Reflex to HIV-1 Drug Resistance by Next Generation Sequencing
Also known as: HIV QT GR
Use
This test is used to detect and quantify HIV-1 RNA in plasma, which aids in the clinical management of HIV-1 infected patients. It helps assess patient prognosis by measuring the baseline HIV-1 RNA levels and monitors the effects of antiretroviral therapy by observing changes in these levels. The test also determines HIV-1 drug resistance using DNA sequencing, which informs treatment decisions to improve therapy outcomes.
Special Instructions
The test requires the collection of plasma, which should be separated from cells within 24 hours and transferred to a standard transport tube. It is important to ship the sample in a frozen state. The commercial pathways include clinical, provider-ordered, and institution/client-billed options, with additional charges applicable if the reflex testing to HIV-1 Drug Resistance by Next Generation Sequencing is performed.
Limitations
The test should not be used for blood donor screening, associated reentry protocols, or for screening human cells, tissues, and cellular- or tissue-based products (HCT/P). A result of 'Not Detected' does not rule out the presence of inhibitors or an HIV-1 RNA concentration below the detection level. The performance characteristics depend on factors such as patient adherence, percentage of resistant virus population, dosing, and drug pharmacology.
New York Approved
Methodology
PCR-based
Biomarkers
LOINC Codes
- 20447-9
- 25835-0
- 29541-0
Result Turnaround Time
2-14 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
4 mL
Minimum Volume
2.5 mL
Container
ARUP standard transport tube (ARUP supply #15824)
Collection Instructions
Separate from cells within 24 hours of collection. Transfer 4 mL plasma to a standard transport tube available through ARUP supply.
Storage Instructions
Ship FROZEN
Causes for Rejection
Serum and heparinized specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 72 hours |
| Frozen | 3 months |