Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Graphical) With Reflex to PhenoSense Entry®
Also known as: HIV-1 Plasma Viremia, HIV-1 RNA by Real-time (RT) PCR, Quantitative, Reflex to PhenoSense Entry®, Viral Load
Use
Detect and quantitate HIV-1 in plasma with reflex to phenotype (at additional charge) if viral load is >1000 copies/mL.
Special Instructions
The test requires careful specimen handling to avoid delays in processing. Plasma must be collected in specific tubes and processed within a short timeframe to ensure accuracy. Additionally, separate frozen specimens are required for each requested test to ensure proper analysis.
Limitations
The assay has a quantitation range of 20 to 10,000,000 copies/mL, and it cannot detect or quantify HIV-1 RNA outside of this range. Incorrect specimen collection or handling, such as the use of green-top heparin tubes or improper tube types, can lead to inaccurate results. Additionally, only frozen samples can be used for specific parts of the test like the PhenoSense Entry® assay to maintain sample integrity.
Methodology
PCR-based (qPCR)
Biomarkers
LOINC Codes
- 20447-9
- 29541-0
- 80563-0
- 51969-4
- 82719-6
- 77202-0
Result Turnaround Time
5-7 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
6 mL
Minimum Volume
4 mL
Container
screw-cap polypropylene frozen transport tubes
Collection Instructions
Collect specimen in lavender-top (EDTA) or PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Remove plasma, transfer plasma to screw-cap polypropylene transport tubes (not "pop-top" or "snap-cap"), and freeze. Ship frozen (preferred). Do not ship frozen in PPT™. If PPT™ is used to collect plasma, specimen must be transferred to screw-cap tubes prior to freezing to avoid false elevation of low viral load samples.
Storage Instructions
Freeze. Separate frozen sample required for PhenoSense Entry® portion of the test. Frozen sample is preferred for the viral load assay but is also stable at room temperature for one day or refrigerated for five days. Freeze for long-term storage.
Causes for Rejection
Hemolysis; green-top (heparin) tube; specimen received in "pop-top" or "snap-cap" tube; quantity not sufficient for analysis; frozen PPT™
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 1 day |
| Refrigerated | 5 days |