Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Graphical) With Reflex to HIV-1 GenoSure PRIme® or GenoSure Archive®
Also known as: HIV-1 Plasma Viremia, HiV-1 RNA Real-time PCR, Quantitative, Reflex to GenoSure Archive®, Reflex to GenoSure PRIme®, Viral Load
Use
Detect and measure HIV-1 in plasma with reflex to GenoSure PRIme® if viral load is greater than or equal to 500 copies/mL or reflex to GenoSure Archive® if viral load is less than 500 copies/mL. The GenoSure PRIme® and GenoSure Archive® assays detect mutations in the HIV viral genes that encode for HIV protease, reverse transcriptase and integrase. Mutations in these genes have been associated with resistance to antiretroviral drugs.
Special Instructions
Not provided.
Limitations
This procedure should only be used for patients with a documented HIV-1 infection. The results should not be used as the sole criteria for patient management. The test was developed and its performance characteristics determined by Monogram Biosciences and has not been cleared or approved by the FDA.
Methodology
PCR-based (Real-time PCR)
Biomarkers
LOINC Codes
- 20447-9 - HIV1 RNA # SerPl NAA+probe
- 20447-9 - HIV1 RNA # SerPl NAA+probe
- 29541-0 - HIV1 RNA SerPl NAA+probe-Log#
- 80563-0 - Report
- 80693-5 - HIV1 provir DNA RT + PR + IN Mut Det Seq
- 80694-3 - HIV1 proviral DNA Gentyp Bld MC Nar
- 51969-4 - Gene analysis narr rpt Doc
- 80689-3 - HIV 1 RNA RT + PR + IN Mut Det Plas Seq
- 62364-5 - Test performance info Spec
- 80695-0 - HIV 1 proviral DNA Gentyp Bld MC Doc
- 8251-1 - Service Cmnt-Imp
Result Turnaround Time
4-6 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
5 mL
Minimum Volume
3.5 mL
Container
Lavender-top (EDTA) tube, screw-capped polypropylene frozen transport tube
Collection Instructions
Both frozen plasma and frozen whole blood are required. Draw three lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Plasma: Centrifuge two tubes within six hours of collection. Remove plasma, transfer to transport tubes, and freeze. Ship frozen.
Storage Instructions
Freeze.
Causes for Rejection
Insufficient volume; heparinized plasma; specimen not frozen
Stability Requirements
| Temperature | Period |
|---|---|
| Frozen | 6 weeks |
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