Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Nongraphical) With Reflex to HIV-1 GenoSure PRIme®
Also known as: HIV-1 Plasma Viremia, HIV-1 RNA Real-time PCR, Quantitative, Reflex to GenoSure PRIme®, Viral Load
Use
Detect and measure HIV-1 in plasma with reflex to GenoSure PRIme® if viral load is ≥500 copies/mL. GenoSure PRIme® assay detects mutations in the HIV viral genes that encode for HIV protease, reverse transcriptase and integrase. Mutations in these genes have been associated with resistance to antiretroviral drugs.
Special Instructions
This procedure is intended for one-time use only and does not provide serial monitoring. If serial monitoring is required, please order test 550630. A separate frozen specimen is required for each test requested.
Limitations
The assay range is from 20 to 10,000,000 copies/mL. The test does not allow for repeat testing if the minimum volume is submitted. Hemolysis, use of green-top (heparin) tubes, nonfrozen specimens, specimens exposed to repeated freeze/thaw cycles, and insufficient quantity for analysis can lead to specimen rejection.
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 20447-9
- 20447-9
- 29541-0
- 8251-1
- 80689-3
- 62364-5
Result Turnaround Time
15-21 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
6 mL
Minimum Volume
4 mL
Container
Lavender-top (EDTA) or plasma preparation tubes (PPT™)
Collection Instructions
Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Transfer plasma to one or more screw-cap polypropylene transport tube(s). Snap-cap or pop-top tubes should be avoided and may result in specimen rejection. Immediately freeze specimen at -20°C. Do not place samples on dry ice until completely frozen. Ship specimens frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Storage Instructions
Freeze. A separate frozen sample is required for GenoSure PRIme portion of the test. Frozen sample preferred for viral load assay but also stable at room temperature for one day or refrigerated for five days. Freeze for long-term storage.
Causes for Rejection
Hemolysis; green-top (heparin) tube; nonfrozen specimens; specimens exposed to repeated freeze/thaw cycles; quantity not sufficient for analysis; frozen PPT™