Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Nongraphical) With Reflex to HIV-1 GenoSure® MG
Also known as: HIV-1 Plasma Viremia, HIV-1 RNA by Real-time PCR, Quantitative, Reflex to Genotype, Viral Load
Use
Detect and quantitate HIV-1 in plasma with reflex to genotype (at additional charge) if viral load is >500 copies/mL. GenoSure® MG test detects mutations in the HIV viral genes that encode the HIV protease and polymerase (reverse transcriptase). Mutations in these genes have been associated with resistance to antiretroviral drugs.
Special Instructions
This procedure is intended for one-time use only and does not provide serial monitoring. If serial monitoring is required, a different test (550422) should be ordered.
Limitations
The assay range for this test is between 20 to 10,000,000 copies/mL. It doesn't support serial monitoring, making it essential to order the specified alternative for ongoing monitoring needs.
Methodology
PCR-based (Real-time PCR)
Biomarkers
LOINC Codes
- 20447-9
- 20447-9
- 29541-0
- 8251-1
Result Turnaround Time
5-13 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
6 mL
Minimum Volume
4 mL
Container
Screw-cap polypropylene frozen transport tube
Collection Instructions
Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Transfer plasma to one or more screw-cap polypropylene transport tube(s). Avoid 'snap-cap' or 'pop-top' tubes as they may result in specimen rejection. Immediately freeze specimen at -20°C. Ship specimens frozen. Do not place samples on dry ice until completely frozen.
Storage Instructions
Freeze. Stable at room temperature for one day or refrigerated for five days. Freeze for long-term storage.
Causes for Rejection
Hemolysis; green-top (heparin) tube; quantity not sufficient for analysis; frozen PPT™
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 1 day |
| Refrigerated | 5 days |