MGMT (O6-Methylguanine-DNA Methyltransferase) Gene Methylation Assay
Also known as: O6-Methylguanine-DNA Methyltransferase (MGMT)
Use
MGMT (O6-methylguanine-DNA methyltransferase) is a DNA repair enzyme that is involved in the repair of damage caused by a variety DNA cross-linking compounds, including alkylating agents. Increased methylation of the MGMT gene promoter region causes diminished or silenced expression of the gene, making cells more sensitive to DNA damage. The relationship has been shown for glioblastomas and alkylating agents such as temozolomide (Temodar®).
Special Instructions
Not provided.
Limitations
Preparation of DNA from tissue samples depends on the quality of the provided specimen. Inadequate DNA extraction may occur in a significant number of paraffin-embedded samples.
Methodology
PCR-based (Methylation-specific PCR)
Biomarkers
No genes
Gene
LOINC Codes
- 60252-4 - MGMT gene methylation Tiss Ql
- 60252-4 - MGMT gene methylation Tiss Ql
- 64083-9 - MGMT methylation score Tiss
- 77202-0 - Laboratory comment Report
- 49549-9 - Ref lab test method
- 72486-4 - Lab director name Provider
- 8100-0 - Specimen preparation
Result Turnaround Time
7-10 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
FFPE tissue block or eight unstained slides at 5 μM and one matching H&E slide or nine precut unstained slides from a paraffin block in 5-μM sections
Minimum Volume
50mm2 total tumor area is required
Collection Instructions
FFPE tissue block or eight unstained slides at 5 μM and one matching H&E slide or nine precut unstained slides from a paraffin block in 5-μM sections
Storage Instructions
Maintain blocks/slides at room temperature.
Causes for Rejection
Tissue is not primary brain tumor; tumor block containing insufficient tumor tissue; tumor fixed in a heavy metal fixative; broken or stained slides; decalcified specimens
Other tests from different labs that may be relevant