Casandra

Casandra Test Code LC17687Version 1 (DRAFT)

Performing Lab

Lab LabcorpLab Test ID 117866CPT Codes 85210, 85220, 85230, 85260, 85384, 85705

Prolonged Protime Profile

Clinical Use

Order Test

Use

Determine the cause of an extended prothrombin time

Special Instructions

Not provided.

Limitations

The test's accuracy and reliability depend on proper specimen collection and handling. Underfilled or improperly mixed tubes may lead to incorrect results. Variations in sodium citrate concentration can affect test outcomes, so adherence to guidelines is crucial. The presence of lupus anticoagulant or other interfering substances might affect the dilute prothrombin time measurement. The test does not detect all possible coagulation disorders and should be considered in conjunction with other clinical and laboratory findings for comprehensive assessment of bleeding disorders.

Test Details

Methodology

Other

Biomarkers

7 components

Dilute Prothrombin TimedPT Confirm RatioFibrinogen ActivityFactor II Activity+3 more

LOINC Codes

Result Codes

75508-2 - Confirm aPTT/normal
75884-7 - aPTT screen to confirm ratio
3255-7 - Fibrinogen PPP-mCnc
3289-6 - Prothrom Act/Nor PPP
3193-0 - Fact V Act/Nor PPP
3198-9 - Fact VII Act/Nor PPP
3218-5 - Fact X Act/Nor PPP

Result Turnaround Time

2-3 days

Specimen

Plasma

Volume

3 mL

Minimum Volume

2 mL

Container

Blue-top (sodium citrate) tubes

Collection Instructions

Citrated plasma samples should be collected by double centrifugation. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio. The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples, except when using a winged blood collection device (ie, "butterfly"), in which case a discard tube should be used. When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge for 10 minutes and carefully remove 2/3 of the plasma using a plastic transfer pipette, being careful not to disturb the cells. Deliver to a plastic transport tube, cap, and recentrifuge for 10 minutes. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a Labcorp PP transpak frozen purple tube with screw cap (Labcorp No. 49482). Freeze immediately and maintain frozen until tested.