Reptilase Time
Use
Diagnosis of fibrinogen deficiency, both congenital and acquired, as well as screen for dysfibrinogenemia.6 Normal values for reptilase time when thrombin time is prolonged suggests the presence of heparin or a direct thrombin inhibitor.
Special Instructions
Reptilase is a thrombin-like enzyme that plays a critical role in evaluating test plasma for the presence of heparin, heparinoids, or hirudin, as reptilase is not affected by these substances.
Limitations
This is a qualitative assay of fibrinogen function, and it may not always indicate a specific disorder. Therefore, additional testing is recommended to clarify results if prolongation is observed. Factors such as liver disease, renal disease, and disseminated intravascular coagulation (DIC) can impact results.
Methodology
Other
Biomarkers
LOINC Codes
- 6683-7
- 6683-7
Result Turnaround Time
2-3 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1 mL
Minimum Volume
Not provided
Container
Blue-top (sodium citrate) tube
Collection Instructions
Citrated plasma samples should be collected by double centrifugation, mixed immediately by gentle inversion, and centrifuged again before freezing.
Patient Preparation
Ideally, the patient should avoid anticoagulant therapy such as warfarin (Coumadin®) for two weeks, and heparin, direct Xa, and thrombin inhibitor therapies for about three days prior to testing.
Storage Instructions
Freeze immediately and maintain frozen until tested.
Causes for Rejection
Severe hemolysis; improper labeling; clotted specimen; specimen diluted with IV fluids; samples thawed in transit; improper sample type; sample out of stability
Stability Requirements
| Temperature | Period |
|---|---|
| Frozen | 28 days |