Hepatitis A Antibody, Total with Reflex to IgM
Use
Detection of total antibodies against Hepatitis A Virus (HAV) may indicate prior immunization or resolved infection; if total antibody is reactive, reflex testing for IgM antibodies is performed to assist in identifying acute infection. Antibody patterns assist in differentiating immune status, recent/remote infection versus vaccination.
Special Instructions
Supplements containing biotin may interfere with assay results. For routine biotin dosing, collect sample at least 8 hours after last dose; for high-dose biotin supplementation, wait at least 72 hours before collection. Reflex IgM testing incurs additional charge.
Limitations
Interference from biotin supplementation; performance for immunocompromised patients, cord blood, patients under age 2, or cadaveric specimens may not be established.
Methodology
Immunoassay (Other)
Biomarkers
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.5 mL
Container
Plastic screw-cap vial; serum from 1 mL serum; EDTA plasma also acceptable
Patient Preparation
Avoid biotin interference—see special notes
Causes for Rejection
Gross hemolysis; gross lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 30 days |