Kappa/Lambda Light Chains, Free with Ratio, Random Urine
Also known as: Immunoglobulin Free Light Chains
Use
Measurement of free kappa and lambda light chains in random (or 24‑hour) urine, and calculation of their ratio, aids in the evaluation and monitoring of monoclonal gammopathies such as multiple myeloma, primary amyloidosis, light chain deposition disease, and related plasma cell disorders. It is used in diagnosis, prognosis, disease activity monitoring, and assessing response to therapy.
Special Instructions
Collect a random urine specimen (preferred) or 24‑hour urine (no preservatives). Submit 2 mL (minimum 1 mL) aliquot in sterile screw‑cap transport container. If timed urine, indicate total volume on specimen, accession comments, and batch sheet.
Limitations
Specimen must be free of gross hemolysis or microbial contamination. No preservatives should be used. Limitations inherent to immunoturbidimetric methods apply (e.g., interference by high protein concentrations or turbidity).
Methodology
Immunoassay (Immunoturbidimetry)
Biomarkers
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Urine
Volume
2 mL
Minimum Volume
1 mL
Container
Sterile screw‑cap transport container
Collection Instructions
Random (preferred) or 24‑hour urine; no preservatives; if timed collection, indicate total volume on specimen and documentation.
Causes for Rejection
Gross hemolysis; microbial contamination
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 21 days |
| Frozen | 30 days |