Basement Membrane Zone and Cell Surface (Epithelial) Antibodies, IgG and IgA by IIF

Also known as: EPITHELIAL

Clinical Use

Use

The Basement Membrane Zone and Cell Surface (Epithelial) Antibodies, IgG and IgA by IIF test is used for indirect immunofluorescence monitoring of linear IgG and IgA basement membrane zone antibody-associated diseases or IgG and IgA cell surface/intracellular substance antibody-associated diseases. It is particularly useful in cases where ELISAs may not show increased antibody levels. This test assesses changing patterns of epithelial antibody expression and is not the preferred initial test for immunobullous diseases but can provide a broad epithelial antibody assessment when used in conjunction with other specific ELISA tests.

Special Instructions

Not provided.

Limitations

The test is not as sensitive as ELISAs for certain antibody detections and monitoring, as ELISAs are restricted to antibodies with specificity to the epitopes included in the test. Negative results in this assay do not exclude the presence of antibodies associated with basement membrane zone or cell surface protein conditions. Additionally, the test results may not rule out pemphigus, pemphigoid, or epidermolysis bullosa acquisita, and clinical correlation is necessary.

Test Details

New York Approved

Methodology

Immunoassay (Indirect Imunofluorescence)

Biomarkers

IgA

Protein

IgA antibody

Other • Binary (Detected / Not Detected)

IgG

Protein

IgG antibody

Other • Binary (Detected / Not Detected)

IgG4

Protein

IgG4 antibody

Other • Binary (Detected / Not Detected)

LOINC Codes

Result Codes

66877-2 - Skin Ab pattern Ser-Imp

Result Turnaround Time

3-9 days

Specimen

Serum

Volume

2 mL

Minimum Volume

0.5 mL

Container

ARUP standard transport tube

Collection Instructions

Collect in plain red or serum separator tube (SST).

Storage Instructions

Store refrigerated unless shipping within 2 hours. Transport at ambient temperature to arrive within 7 days. If 7-14 days until received, store and ship refrigerated. If greater than 14 days, store and ship frozen.