Direct Coombs (Anti-Human Globulin)
Also known as: IRL-DC
Use
The Direct Coombs test is used to detect in vivo coating of red blood cells (RBCs) with immunoglobulin and complement degradation products on the patient's RBCs. This test is useful for antibody identification and evaluation of transfusion reactions, hemolytic disease of the fetus and newborn (HDFN), and autoimmune hemolytic anemia that may be associated with certain diseases or drugs.
Special Instructions
Not provided.
Limitations
The test may not precisely characterize the type of coating (e.g., IgG or C3d) if the reactivity is too weak. Interpretation requires consideration of clinical context.
New York Approved
Methodology
Other
Biomarkers
In vivo coating of RBCs with immunoglobulin or complement
Other
LOINC Codes
- 13946-9 - Comp+Ig RBC Ql
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5 mL
Minimum Volume
1 mL
Container
Lavender (K2EDTA) or Pink (K2EDTA)
Storage Instructions
Transport refrigerated.
Causes for Rejection
Separator or gel tubes.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | 1 week |
| Frozen | Unacceptable |
Other tests from different labs that may be relevant