Coombs', Direct
Also known as: DAT, Direct Antihuman Globulin Test, Direct Coombs'
Use
Detect in vivo coating of IgG and/or complement components on RBCs
Special Instructions
The test utilizes antiglobulin test methodology. Red cells that test positive are further analyzed with monospecific antisera which include anti-IgG and anticomplement.
Limitations
The presence of potent cold autoagglutinins can interfere with test interpretation. False positives may also occur if clotted blood samples are used, particularly affecting anticomplement results.
Methodology
Immunoassay (Other)
Biomarkers
LOINC Codes
- 1007-4
- 1007-4
- 1006-6
- 13946-9
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
4 mL
Minimum Volume
0.5 mL
Container
Lavender-top (EDTA) tube
Storage Instructions
Specimens should be tested as soon as possible or within 72 hours of collection. Samples that cannot be tested immediately should be stored at 2°C to 8°C. Do not freeze.
Causes for Rejection
Gross hemolysis; bacterial or other contamination; specimen more than five days old