Casandra
Casandra Test Code QD23387Version 1 (DRAFT)
Performing Lab
Direct Antiglobulin Test (DAT) with Reflex to Anti‑C3 and Anti‑IgG
Also known as: Direct Coombs, Coombs Direct
Clinical Use
Order TestUse
The DAT (Direct Coomb’s test) is positive if red cells have been coated in vivo with immunoglobulin, complement, or both. A positive result may indicate immune‑mediated red cell destruction such as autoimmune hemolytic anemia, a transfusion reaction, or drug‑induced hemolysis.
Special Instructions
If submitting with any other test requiring whole blood, please submit a separate whole blood tube for the DAT.
Limitations
Not provided.
Test Details
Methodology
Other
Biomarkers
DAT‑positive indicates coated red cells with immunoglobulin or complement
OtherDAT • Other • Categorical (e.g., Positive / Negative / Indeterminate)
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen Requirements
Specimen
Whole Blood
Volume
5 mL
Minimum Volume
1 mL
Container
EDTA lavender or pink‑top tube or ACD‑A or ACD‑B (yellow‑top) tube
Storage Instructions
Refrigerated
Causes for Rejection
Gross hemolysis, grossly lipemic, serum separator tube, clotted specimen, grossly icteric
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 72 hours |
| Frozen | Unacceptable |