Hemoglobin Evaluation by HPLC With Reflex to Electrophoresis and/or RBC Solubility
Also known as: HGBEL RFX
Use
The Hemoglobin Evaluation by HPLC with Reflex to Electrophoresis and/or RBC Solubility is an effective test for screening hemoglobinopathies, which may include conditions such as thalassemias and unstable hemoglobins. It is also suitable for follow-up in individuals with a known hemoglobinopathy. This screening process aids in identifying variants that cannot be quantitated directly by HPLC, with reflex testing to RBC Solubility or Capillary Electrophoresis for confirmation and identification of the variant.
Special Instructions
Not provided.
Limitations
The test may not detect all possible hemoglobin variants. Normal results from HPLC and capillary electrophoresis do not rule out the possibility of alpha globin gene deletions associated with silent carrier status or alpha thalassemia trait. Additionally, individuals with certain rare thalassemia variants may not be identified by this assay if they exhibit normal Hb A2 levels.
New York Approved
Methodology
Other
Biomarkers
LOINC Codes
- 4547-6 - Hgb A1 MFr Bld
- 4551-8 - Hgb A2 MFr Bld
- 4576-5 - Hgb F MFr Bld
- 4625-0 - Hgb S MFr Bld
- 4563-3 - Hgb C MFr Bld
- 4575-7 - Hgb E MFr Bld
- 48343-8 - Hgb Other MFr Bld
- 21026-0 - Path Interp Bld-Imp
- 6864-3 - Hgb S Bld Ql Soly
- 13514-5 - Hgb Fract Bld Elph-Imp
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5 mL
Minimum Volume
0.5 mL
Container
Lavender (EDTA) or pink (K2EDTA)
Storage Instructions
Refrigerated
Causes for Rejection
Frozen or room temperature specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | 1 week |
| Frozen | Unacceptable |
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