Hemoglobin (Hb) Solubility With Reflex to Hemoglobinopathy Fractionation Cascade, Blood
Also known as: Hb Solubility, Sickle Cell Preparation, Sickle Cell Solubility Test, Sickle Cell Test, Sickle Prep
Use
Qualitative determination of presence of hemoglobin S; detect sickling hemoglobins; evaluate hemolytic anemia, undiagnosed hereditary anemia with morphologic (sickle-like) abnormalities on peripheral blood smear
Special Instructions
This test automatically reflexes to hemoglobin fractionation by HPLC if positive, which incurs additional charges. Ensure a separate lavender-top tube is submitted for each test requiring this specimen type to avoid delays in turnaround time.
Limitations
The limitations are provided in the related test, Hemoglobin (Hb) Solubility [005223], which should be consulted for detailed constraints. This test may not detect all variants of sickle cell hemoglobin or other non-hemoglobin S pathologies without further fractionation.
Methodology
Other
Biomarkers
LOINC Codes
- 6864-3
- 6864-3
- 32682-7
- 20572-4
- 4552-6
- 32683-5
- 12710-0
- 42246-9
- 42244-4
- 42245-1
- 44923-1
- 44920-7
- 44922-3
- 42248-5
Result Turnaround Time
2-3 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
1 mL
Minimum Volume
0.6 mL (Note: This volume does not allow for repeat testing.)
Container
Lavender-top (EDTA) tube; capillary puncture: three microhematocrit tubes
Collection Instructions
To avoid delays in turnaround time, please submit a separate lavender-top tube for each test requiring a lavender-top.
Patient Preparation
No preliminary fluid or food restriction is required.
Storage Instructions
Room temperature
Causes for Rejection
Specimen other than whole blood received; clotted specimen
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |